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KMID : 0381120200420070817
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Genes and Genomics
2020 Volume.42 No. 7 p.817 ~ p.825
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Effects of p-coumaric acid on microRNA expression profiles in SNU-16 human gastric cancer cells
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Jang Mi-Gyeong
Ko Hee-Chul
Kim Se-Jae
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Abstract
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Background: p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16 gastric cancer cells. However, the effects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported.
Objectives: The primary objective of this study was to investigate microRNA expression profiles in p-CA-treated SNU-16 cells.
Methods: SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the differentially expressed microRNAs (DEMs).
Results: Thirty-two DEMs were identified between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identified hsa-miR-125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes.
Conclusions: Our findings indicated that p-CA exerted its anticancer effects in SNU-16 gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.
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KEYWORD
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MicroRNA, SNU-16 cell, p-Coumaric acid, Next-generation sequencing
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